Evaluation of Cowpea (Vigna unguiculata L) Mutant Genotypes under Hydroponic Conditions Using Different Levels of Phosphorus Concentration
Makela, V.M. *
Department of Plant Science, School of Agricultural Sciences, University of Zambia (UNZA), P.O. Box 32379, Great East Road Lusaka, Zambia.
Tembo, L.
Department of Plant Science, School of Agricultural Sciences, University of Zambia (UNZA), P.O. Box 32379, Great East Road Lusaka, Zambia.
Siyunda, C.A.
Department of Plant Science, School of Agricultural Sciences, University of Zambia (UNZA), P.O. Box 32379, Great East Road Lusaka, Zambia, School of Agricultural Sciences, Rockview University, Zambia and Department of Crop Science, Natural Resources Development College (NRDC), CH 99, Chelstone, Lusaka, Zambia.
Mbuma, W.N.
Department of Plant Breeding, Agricultural Research Council, Industrial and Medicinal Plant Institute, Private Bag X293, Pretoria 0001, South Africa and Department of Plant Sciences, Plant Breeding, University of the Free State, P.O Box 339, Bloemfontein 9300, South Africa.
Alamu, O.E.
Food and Nutrition Sciences Laboratory, International Institute of Tropical Agriculture, Southern Africa, Research and Administration Hub (SARAH) Campus P.O Box 310142, Chelstone, Lusaka 10101, Zambia.
Batieno, B.J.
Institut de Environnement et de Recherches Agricole (INERA) 01 BP 476 Ouagadougou 01, Burkina Faso.
Senthilraja, N.
Department of Entomology, B. A. College of Agriculture, Anand Agricultural University, Anand – 388110, Gujarat, India.
Oladipo, M.S.
Institute of Agriculture Research and Training, Moor Plantation, P.M.B. 5029, Ibadan, Nigeria.
Tembo, G.
School of Agricultural Sciences, Rockview University, Zambia.
Chimbila, J.W.
School of Agricultural Sciences, Rockview University, Zambia.
*Author to whom correspondence should be addressed.
Abstract
Cowpea yields in the sub-Sahara African region are low primarily due to low available soil health, poor varieties and farming management. The present study aims to assess the performance of mutant genotypes of cowpea (Vigna unguiculata L) under hydroponic conditions, specifically focusing on different levels of phosphorus concentration. This study was conducted at the University of Zambia, School of Agricultural Sciences, plant physiology laboratory. The experiment was set up as a 5 x 8 factorial completely randomized design replicated three times with varying P amounts of 0mg/L, 4mg/L, 8mg/L, 12mg/L, and 16mg/L in a nutrient media and eight genotypes, giving a total of 120 experimental units assigned randomly to each plot (black plastic container). Genotypes showed significant differences (p<0.001) in response to all measured parameters (shoot length, root length, lateral roots, shoot biomass, and root biomass) across phosphorus concentrations. Similarly, the responses of all measured parameters in different P concentrations across genotypes were significantly different (P < 0.001). The current study identified the mutant line LT-3-8-4-1 as a better performer in the variables under consideration across different P-concentrations. However, mutant lines LT-3-8-4-1, LT-11-5-1-1, and LT-4-2-4-1 were identified as the best performers in the P-limiting solution (with P amount at 4mg/L). Furthermore, mutant line LT-3-8-4-1 exhibited a better adaptive flexibility performance than the parent (Lutembwe) in media with a P concentration ranging from 0 to 16 mg/L while the three genotypes, LT-3-8-4-1, LT-11-5-1-1, and LT-4-2-4-1 were considered as genotypes of choice in areas where there are p-limiting soils. In addition, the phenotypic variation explained showed that only 9.79% of the change in shoot length is explained by the change in root length. This implies that shoot length can only be used to estimate the root length in selecting for genotypic efficiency at utilizing P in the hydroponic medium. Based on the current study's findings, it is recommended that mutant line LT-3-8-4-1 replace the parental line regarding phosphorus use efficiency. Additionally, shoot length can be used to determine what is occurring in the root length as the two parameters are correlated.
Keywords: Cowpea, hydroponic, mutant lines, phosphorus use efficiency